FI93743B - På transskription baserade - Google Patents

DATABLAD FÖR MATERIALSÄKERHET - Gen-Probe, Inc.

The Luminex-based HPV genotyping was a sensitive, reproducible technique for the simultaneous genotyping of all clinically relevant genital HPV types. The aim of this study was to estimate the genotype-specific prevalence of human papillomavirus (HPV) DNA in patients with cervical pathology using the multiplex PCR and Luminex xMAP technology. In addition, we compared genotyping with Luminex xMAP and with Reverse Line Blot (RLB). HPV DNA Array detected CIN2+ lesions with a sensitivity of 90.2%, identical to that of MPG-Luminex.

The detection limit is between 10 and 100 copies for most mucosal HPV types. The aim of this study was to estimate the genotype-specific prevalence of human papillomavirus (HPV) DNA in patients with cervical pathology using the multiplex PCR and Luminex xMAP technology. In addition, we compared genotyping with Luminex xMAP and with Reverse Line Blot (RLB). Our HPV genotyping assay was developed to permit A set of 19 type-specific HPV RNA probes were prepared rapid and accurate genotyping with a high level of multi- by in vitro transcription of near full-length HPV DNA plex, while avoiding or mitigating the stated drawbacks of clones.

The Luminex‐based HPV genotyping was a sensitive, reproducible technique for the simultaneous genotyping of all clinically relevant genital HPV types. This assay system may be used to provide critical clinical information for early detection of HPV, especially in cases where the HPV copy numbers are low and the latency period of HPV infection is prolonged. Highly Sensitive Detection and Genotyping of HPV by PCR Multiplex and Luminex Technology in a Cohort of Colombian Women with Abnormal Cytology Dabeiba A García 1 , Angel Cid-Arregui * , 2 , Markus Schmitt 3 , Marcos Castillo 4 , 5 , Ignacio Briceño 4 , Fabio A. Aristizábal6 6 high-risk human papillomavirus (HPV) types.

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2018 — Global proficiency study of human papillomavirus genotyping. Eklund En sådan är vår multiplexanalys Pseudovirion-Luminex, där vi tillverkar Human papillomavirus testing and its application in cervical cancer prevention new methods for HPV-testing with genotyping: A high-throughput HPV genotyping probes coupled to fluorescent beads for detection on the Luminex platform.

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HPV 16 on the Luminex xMAP system, also called the HPV DNA suspension array Apr 24, 2014 HPV genotyping. Multiplex PCR. Luminex. Cervical cancer. a b s t r a c t. Regional differences in human papillomavirus (HPV) genotypes and Cobas HPV test, Roche.

The bead-based technology (Luminex suspension array technology) provides a rapid and cost-effective method to simultaneously detect different HPV genotypes. The Luminex-based HPV genotyping was a sensitive, reproducible technique for the simultaneous genotyping of all clinically relevant genital HPV types. This assay system may be used to provide critical clinical information for early detection of HPV, especially in cases where the HPV copy numbers are low and the latency period of HPV infection is prolonged.
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Explore the latest full-text research PDFs, articles, conference papers, preprints and more on LUMINEX. Find methods information, sources, references or conduct a literature review on LUMINEX Lyme Disease Assays HPV Genotyping Kit The System Luminex® xMAP® technology 3 Consumables 5 Lyme Disease Assays Optiplex Borrelia IgG and IgM 6 Lyme Disease Assays Optiplex Borrelia Screening IgG and IgM 8 HPV Test Optiplex HPV Genotyping Kit 9 Technology Introducing the Luminex® Technology 3 Luminex® xMAP® technology is highly sensitive HPV högrisk typer. METOD/ ANALYSPRINCIP Efter nukleinsyraextraktion av provmaterialet utförs PCR-amplifiering med hjälp av av HPV primers med förmåga att detektera ett brett spektrum av HPV hög- och lågrisk typer. PCR-produkten analyseras därefter så kallad Luminex-teknik för identifiering av HPV typ(er).

▫ Probes for 42 different HPV-types and two universal probes. ▫ Valid result: ▫ Blank-block: HPV-antikropps test (Luminex) testar för antikroppar mot 30 HPV-typer. • HPV- neutralisationstest HPV DNA genotyping proficiency panel. Blindade paneler in this study indicate that the Luminex multiplex assay tested has the potential to be used routinely for screening flocks.
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Find methods information, sources, references or conduct a literature review on GENOTYPING multiple HPV infections (median OR=4.6,CI 95%=1.8-11.7). Conclusions: A multiplexed HPV PsV-Luminex assay has been developed and validated to correlate with natural HPV infection for 13 HPV types, thus enabling more comprehensive studies in HPV epidemiology and vaccine research. Nazarenko I, Kobayashi L, Giles J, Fishman C, Chen G, Lorincz A: A novel method of HPV genotyping using Hybrid Capture sample prep- We thank Nina Brown, Kim Keating, Sameera Rangwala, aration method combined with GP5⫹/6⫹ PCR and multiplex detec- Hiam Salim, Julie Giles, and others in R&D at QIAGEN for tion on Luminex XMAP. Become a research or clinical partner of Luminex Corporation and be part of a mutually rewarding partnership with market leaders. Luminex ffpe blocks Ffpe Blocks, supplied by Luminex, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations.

Consecutive HPV genotyping of invasive cervical - Acces

• Luminex LX100 or LX200 • Vacuum Wash Station • Vortex • 8-Channel Pipette, (5-100 µL) VII. Laboratory Organization In order to prevent contaminations of genomic DNA samples we highly recommend to perform the indivi-dual operations of the HPV genoptyping kit in separate laboratory areas (please ensure a 3-room-solution), as The multiplex Luminex hybridization assay enabled the detection of 27 mucosal HPV-genotypes: i) high risk (HR)-HPV types: 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68; ii) possibly HR-HPV types: 26, 53, 67, 70, 73, 82; low risk (LR)-HPV types: 6, 11, 30, 43, 44 and 69. Multiplex Technologies May Provide a More Accurate Method for HPV Identification. This research shows that multiplexing technology may be used as a successful method for accurately detecting the types of HPV (human papillomavirus). 2014-11-01 Abstract Human papillomavirus (HPV) detection is used for screening of cervical cancer and genotype‐specific persistence has shown to be mandatory for … 2014-08-01 high-risk human papillomavirus (HPV) types. The aim of this study was to estimate the genotype-specific prevalence of human papillomavirus (HPV) DNA in patients with cervical pathology using the multiplex PCR and Luminex xMAP technology. In addition, we compared genotyping with Luminex xMAP and with Reverse Line Blot (RLB). A cohort of Luminex‐based assays for HPV genotyping have proven to be highly sensitive, detecting just 1–10 template copies of plasmid per PCR reaction .

av en mikrosfärisk pärlbaserad analys (Luminex xMAP, Luminex Corp, Austin, TX, hrHPV-typer genotype-överensstämmelse mellan livmoderhalsskrapor och genotype-phenotype correlations in relation to ECG risk factors and diastolic function. This is Luminex analysis of amniotic fluid, vaginal fluid and maternal serum. urinvägssymptom, STI inklusive HPV, och cytologatypier samt cervixcancer. The Luminex-based HPV genotyping was a sensitive, reproducible technique for the simultaneous genotyping of all clinically relevant genital HPV types. All samples analyzed with Luminex xMAP were HPV-positive, while we could detect HPV in only 48.8% of cases with RLB. Of the samples positive by both methods, there was a 67.2% correlation in the viral type(s) detected.